No, Scriptdio Agency is an Upgrade for Scriptdio Standard Monthly, Yearly, or One-Time Subscription.
Once you Upgrade to Scriptdio Agency you can use the Agency features inside your Scriptdio account as long as you keep renewing your Scriptdio standard Monthly or Yearly Subscription. You will not need to pay any additional fee for the Agency Features as long as your Scriptdio Monthly, Yearly, or One-Time Subscription is active.