No, Scriptdio PRO is an Upgrade for Scriptdio Standard Monthly, Yearly, and One-Time Subscription.
Once you Upgrade your Subscription to Scriptdio PRO you can use the PRO Frameworks inside your Scriptdio App as long as you keep renewing your Scriptdio standard Monthly or Yearly Subscription. You will not need to pay any additional fee for the PRO upgrade as long as your Scriptdio Monthly, Yearly or One-Time Subscription is active.